Kano Pillars Wednesday were sent crashing from the Federation Cup by Prime FC, who won 2-1 to advance to the round of 16.
Another lower league side Crown FC beat Niger Tornadoes 5-4 on penalties after both teams were deadlocked at 1-1 at the end of regulation time.
However, other Nigeria premier league sides advanced to the next stage of the cup competition.
Sunshine Stars outscored Abia Comets 4-2 on penalties after full time scores stood at 1-1, Nasarawa stopped Go Round FC 1-0, Rivers United thumped Kaduna United 4-0 and Shooting Stars went past Almar FC 3-0.
RESULTS OF FEDERATION CUP MATCHES
Sunshine Stars 1 Abia Comets 1 (Sunshine win 4-2 on penalties)
Nasarawa United 1 Go Round FC 0
Rivers United 4 Kaduna United 0
Shooting Stars 3 Almar FC 0
Crown FC 1 Niger Tornadoes 1 (Crown win 5-4 on penalties)
ABS FC Vs Enugu Rangers (Abandoned, due to heavy downpour; second half to be played on Thursday morning. Rangers was leading 1-0))
Kogi United 1 Wikki Tourists 2
Kano Pillars 1 Prime FC 2
Warri Wolves 3 Jigawa Stars 0 (W/O)
FC IfeanyiUbah 1 Lobi Stars 0