Frenchman Michel Dussuyer resigned as Guinea coach following their quarter-final elimination by Ghana from the CAF Africa Cup of Nations in Equatorial Guinea.
Guinea were outclassed 3-0 by Ghana in Sunday's quarter-final and had drawn all three of their group matches.
The 55-year-old had been in the job since 2010, and on Sunday had refused to even mull the question of stepping down.
Guinea had made it through to the last eight after a drawing of lots was needed to separate them from Mali, with whom they shared the same points, goals scored and goal difference.